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hepatocellular carcinoma cells  (ATCC)


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    Structured Review

    ATCC hepatocellular carcinoma cells
    Hepatocellular Carcinoma Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+ca/AN3+CA+Quantitative%3B+purified+DNA%2C+3+ug%3B+Human/us12577225-1167-5-11
    Average 94 stars, based on 18 article reviews
    hepatocellular carcinoma cells - by Bioz Stars, 2026-10
    94/100 stars

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    Cell Culture:

    Article Title: M 6 A-modified lncRNA HULC promotes osteosarcoma progression by stabilizing GLUT1 mRNA through interaction with TAF15.
    Article Snippet: .. AR TIC LE IN PR ES S ARTICLE IN PRESS Human OS cell lines, including U2OS and HOS (ATCC, Manassas, VA, USA), were cultured in McCoy's 5A medium (Gibco, Carlsbad, CA, USA) or EMEM (Gibco), supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin, respectively. ..

    Article Title: Loss of cold shock domain-containing protein E1 (CSDE1) function enhances mRNA stability of interleukin-6 and promotes malignant transformation in endometrial cancer cells.
    Article Snippet: .. Cell culture and lentiviral infection Human EC cell lines (Ishikawa, KLE, HEC-1A [ATCC Cat# HTB-112, RRID: CVCL_0293], SPEC-2, AN3CA and HEC-1B [ATCC Cat# HTB-113, RRID: CVCL_0294]) and 293T cells were maintained in our laboratory (purchased from the Chinese Academy of Sciences) and cultured in DMEM supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin, and 100 μg/mL streptomycin at 37 °C in a humidified incubator with 5% CO2. ..

    Modification:

    Article Title: Analytical performance and clinical usability of a low-cost vaginal swab for sensitive detection of high-risk human papillomavirus
    Article Snippet: To minimize contamination risk, plasmids were diluted in Tris-EDTA (TE) buffer, pH 8.0 (Fisher Scientific, Waltham, MA, USA) to a final concentration between 1 and 10 ng/μL and stored at −20°C. .. Human cervical cancer cell lines including SiHa (ATCC HTB-35), HeLa (ATCC CCL-2), CaSki (ATCC CRL-1550), and C-33A (ATCC HTB-31) cells from American Type Culture Collection (ATCC, Manassas, VA, USA) were grown at 37°C with 5% CO 2 in culture media: Dulbecco’s Modified Eagle Medium high glucose with sodium pyruvate containing 10% Fetal Bovine Serum (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) and 50 μg/mL gentamycin (Sigma-Aldrich, St. Louis, MO, USA) under standard aseptic conditions while screening for mycoplasma contamination using the Universal Mycoplasma Detection kit (ATCC, Manassas, VA, USA) according to the manufacturer’s instructions. .. Either the Steripack XpressCollect flocked nylon swabs or Copan FLOQSwabs were contrived using different concentrations of plasmid DNA or human cervical cancer cell lines (shown above).

    Screening:

    Article Title: Analytical performance and clinical usability of a low-cost vaginal swab for sensitive detection of high-risk human papillomavirus
    Article Snippet: To minimize contamination risk, plasmids were diluted in Tris-EDTA (TE) buffer, pH 8.0 (Fisher Scientific, Waltham, MA, USA) to a final concentration between 1 and 10 ng/μL and stored at −20°C. .. Human cervical cancer cell lines including SiHa (ATCC HTB-35), HeLa (ATCC CCL-2), CaSki (ATCC CRL-1550), and C-33A (ATCC HTB-31) cells from American Type Culture Collection (ATCC, Manassas, VA, USA) were grown at 37°C with 5% CO 2 in culture media: Dulbecco’s Modified Eagle Medium high glucose with sodium pyruvate containing 10% Fetal Bovine Serum (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) and 50 μg/mL gentamycin (Sigma-Aldrich, St. Louis, MO, USA) under standard aseptic conditions while screening for mycoplasma contamination using the Universal Mycoplasma Detection kit (ATCC, Manassas, VA, USA) according to the manufacturer’s instructions. .. Either the Steripack XpressCollect flocked nylon swabs or Copan FLOQSwabs were contrived using different concentrations of plasmid DNA or human cervical cancer cell lines (shown above).

    Infection:

    Article Title: Loss of cold shock domain-containing protein E1 (CSDE1) function enhances mRNA stability of interleukin-6 and promotes malignant transformation in endometrial cancer cells.
    Article Snippet: .. Cell culture and lentiviral infection Human EC cell lines (Ishikawa, KLE, HEC-1A [ATCC Cat# HTB-112, RRID: CVCL_0293], SPEC-2, AN3CA and HEC-1B [ATCC Cat# HTB-113, RRID: CVCL_0294]) and 293T cells were maintained in our laboratory (purchased from the Chinese Academy of Sciences) and cultured in DMEM supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin, and 100 μg/mL streptomycin at 37 °C in a humidified incubator with 5% CO2. ..



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